In vitro Anti-MDR Bacteria, Anti-inflammatory and Antioxidant Activities of Ceratonia siliqua L.

Mustafa Shuaib; Ahmed Kabbashi; Salwa Eltawaty; Amar Ismail

doi:10.54361/ajmas.258248

Authors

Mustafa Shuaib Department of Microbiology and Parasitology, Medicinal and Aromatic Plants and Traditional Medicine Research Institute, National Center for Research, Khartoum, Sudan https://orcid.org/0009-0007-9274-2229
Ahmed Kabbashi Department of Biomedical Science, Faculty of Pharmacy, Omar Al-Mukhtar University, Al-Bayda, Libya. Department of Microbiology and Parasitology, Medicinal and Aromatic Plants and Traditional Medicine Research Institute, National Center for Research, Khartoum, Sudan. Department of Microbiology, Faculty of Medical Laboratory Sciences, International University of Africa, Khartoum, Sudan. https://orcid.org/0000-0003-3439-096X
Salwa Eltawaty Department of Biomedical Science, Faculty of Pharmacy, Omar Al-Mukhtar University, Al-Bayda, Libya https://orcid.org/0000-0001-8955-4138
Amar Ismail Department of Biomedical Science, Faculty of Pharmacy, Omar Al-Mukhtar University, Al-Bayda, Libya. Department of Biochemistry and Molecular Biology, Faculty of Science and Technology, Al-Neelain University, Khartoum, Sudan https://orcid.org/0000-0003-0172-8362

DOI:

https://doi.org/10.54361/ajmas.258248

Keywords:

C. siliqua, Antibacterial, MDR Isolates, Anti-Inflammatory, Antioxidant.

Abstract

The present study demonstrated the in vitro antibacterial activity against multidrug-resistant (MDR) isolates, as well as the anti-inflammatory and antioxidant effects of the Ceratonia siliqua L. (C. siliqua) ethanolic extract from Al-Bayda City, located in the Green Mountain. The C. siliqua fruit extract was obtained using 75% ethanol. The agar-well diffusion method investigated the antibacterial activity against MDR isolates. The albumin denaturation method was employed to demonstrate the anti-inflammatory activity, and the antioxidant activity of the extract was evaluated using DPPH scavenging activity. The extract showed promising antibacterial activity against MDR isolates, with higher inhibition zones against S. aureus, followed by Ps. aeruginosa, K. pneumoniae, and E. coli. The extract showed high inhibitory activity against albumin denaturation, with an IC₅₀ of 368.5 µg/ml. Moreover, the DPPH scavenging method revealed a weaker antioxidant activity of the C. siliqua extract (42.86 ± 0.08 %) at a concentration of 500 µg/ml compared with the control. In summary, the fruit C. siliqua ethanolic extract is a promising therapeutic agent for MDR isolates and has the potential to reduce inflammation and oxidative stress.