Prevalence of Methicillin-Resistant Staphylococcus aureus and Their Antibiotic Susceptibility Test Among Diabetic Foot Infection Patients in Misurata City, Libya

Mohanned Al-Washaish; Nabil Elmahaishi; Asma Abu Zayan; Eman Al-Zawawi; Elham Al-Maliki

doi:10.54361/ajmas.258348

Authors

Mohanned Al-Washaish Department of Biomedical Sciences, Faculty of Pharmacy, University of Misurata, Misurata, Libya https://orcid.org/0000-0002-6869-207X
Nabil Elmahaishi Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Misurata, Misurata, Libya https://orcid.org/0000-0001-5585-2786
Asma Abu Zayan Department of Biomedical Sciences, Faculty of Pharmacy, University of Misurata, Misurata, Libya https://orcid.org/0000-0001-7975-0848
Eman Al-Zawawi Department of Biomedical Sciences, Faculty of Pharmacy, University of Misurata, Misurata, Libya https://orcid.org/0000-0001-8754-2459
Elham Al-Maliki Department of Biomedical Sciences, Faculty of Pharmacy, University of Misurata, Misurata, Libya https://orcid.org/0000-0003-0023-2832

DOI:

https://doi.org/10.54361/ajmas.258348

Keywords:

Diabetic Foot Infection, Antibiotic Resistance, MRSA, MSSA.

Abstract

Diabetic foot infection (DFI) is a serious and common complication of diabetes mellitus. This study aimed to determine the prevalence of Methicillin-Resistant Staphylococcus aureus (MRSA) and assess the antibiotic resistance patterns of these isolates among DFIs. A total of 40 samples were collected, isolated, and identified using standard microbiological procedures and biochemical tests. Antibiotic susceptibility testing was carried out using the Disc Diffusion Method. The most common bacterial species detected was Acinetobacter spp. (30%), followed by Staphylococcus aureus (17.5%), Pseudomonas aeruginosa (17.5%), and Klebsiella spp. (15%), CONS (Coagulase-Negative Staphylococci) (15%), and Proteus spp. (5%). Out of the 7 isolates of S. aureus, 71.4% were MRSA, while 28.6% were MSSA (Methicillin-Sensitive Staphylococcus aureus). MRSA isolates showed a high rate of resistance to both AMP (Ampicillin) and AMC (Amoxicillin) (100%), followed by resistance to cefotaxime (80%) and ciprofloxacin (60%).